= Processing ROIs = The parameters below are reasonable choices for standard analyses. However, these Wiki pages are not supposed to substitute the [http://www.nmr.mgh.harvard.edu/meg/manuals/MNE-manual-2.6.pdf MNE manual], [http://imaging.mrc-cbu.cam.ac.uk/meg/MEGpapers reading papers], and [http://imaging.mrc-cbu.cam.ac.uk/imaging/ImagersInterestGroup discussions] with more experienced researchers. ROIs in MNE are defined as "label" files. Freesurfer already creates a parcellation of the cortical surface in various regions of interest (see subdirectory "label" in your subjects' MRI directory). These can be converted to label files for MNE. If you've morphed all your individual STC-files to an average brain (called "average" in the example), you can create MNE-labels from these: {{{ #!/bin/sh mne_annot2labels --subject average --parc aparc }}} This will result in files such as "fusiform-lh.label" in the MRI label subdirectory. These are text files that you can read into a text editor etc. Then you can extract ROI information using mne_make_movie. The following script does it for multiple subjects, conditions, labels and both hemispheres. {{{ #!/bin/sh # ## Your variables: datapath='/myMEGdatapath/' # root directory for your MEG data MRIpath='/myMRIdirectory/' # where your MRI subdirectories are outpath='/myoutputdirectory' # where the results should go #condition names as used in file names conds=('cond1' 'cond2' 'cond3') # subjects names used for MRI data subjects=(\ 'Subject1' \ 'Subject1' \ 'Subject1' \ ) # MEG IDs (your directory structure may differ) subj_pre=(\ 'meg10_0001' \ 'meg10_0002' \ 'meg10_0003' \ ) # MEG subdirectories (your directory structure may differ) subj_dir=(\ '100001' \ '100002' \ '100003' \ ) # labels to be processed labels=(\ 'frontalpole'\ 'fusiform'\ 'inferiorparietal'\ 'inferiortemporal'\ 'insula'\ 'lateraloccipital'\ 'lateralorbitofrontal'\ 'lingual'\ 'medialorbitofrontal'\ 'middletemporal'\ 'paracentral'\ 'parahippocampal'\ 'parsopercularis'\ 'parsorbitalis'\ 'parstriangularis'\ 'pericalcarine'\ 'postcentral'\ 'posteriorcingulate'\ 'precentral'\ 'precuneus'\ 'rostralanteriorcingulate'\ 'rostralmiddlefrontal'\ 'superiorfrontal'\ 'superiorparietal'\ 'superiortemporal'\ 'supramarginal'\ 'temporalpole'\ 'transversetemporal'\ ) hemispheres=(\ 'lh' \ 'rh' \ ) # Processing: nsubjects=${#subjects[*]} lastsubj=`expr $nsubjects - 1` nconds=${#conds[*]} lastcond=`expr $nconds - 1` nlabels=${#labels[*]} lastlabel=`expr $nlabels - 1` nhemispheres=${#hemispheres[*]} lasthemisphere=`expr $nhemispheres - 1` # Processing: for ss in `seq 0 ${lastsubj}` do echo " " echo " Computing movies for SUBJECT ${subjects[m]}" echo " " for cc in `seq 0 ${lastcond}` do echo " " echo " Computing movies for condition ${conds[c]}" echo " " for bb in `seq 0 ${lastlabel}` do echo " " echo " Computing movies for label ${labels[b]}" echo " " for hh in `seq 0 ${lasthemisphere}` do echo " " echo " Computing movies for hemisphere ${hemispheres[h]}" echo " " mne_make_movie \ --subject ${subjects[ss]} \ --stcin ${STCpath}/${subj_pre[m]}_0${subjects[m]}_${conds[c]}_-${hemispheres[h]}.stc \ --label ${MRIpath}/average/label/${labels[b]}-${hemispheres[h]}.label \ --labelverts \ --labeltag -${subjects[m]}_${conds[c]}.amp \ --labeloutdir ${outpath} done # hemispheres done # labels done # conditions done # subjects }}} = Create your own labels = 1) Display a cortical surface (and e.g. an STC file) in mne_analyze 2) Define the borders of your ROI by defining points using your right mouse button 3) Hold the "CTRL" key and click into the centre of your points with your right mouse button 4) Save the label. 5) Use mne_make_movie to extract information for this label. Note that you can use mne_morph_labels to morph labels between subjects, for example to the average brain.