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. MATLAB: tested on versions >=r2009a. (!) In CBSU, you need ''r2012b'' for parallel computing | . MATLAB: tested on versions >=r2009a. (!) In CBSU, you need 'r2012b' for parallel computing |
Automatic analysis (aa)
Automatic analysis (aa) is a pipeline system for neuroimaging, written in Matlab. It supports SPM 5/8 and some functions from FSL.
For more information see:
Version 2.2 and earlier: Manual
- Version 4:
Any problems, report them to Danny Mitchell (version 2 and 3) or Tibor Auer (version 4).
Contents
Paths to aa
Different versions live in:
legacy versions (0.1beta - 2.0) -
unsupported
/imaging/local/spm/aa/aa-ver0.1_beta
/imaging/local/spm/aa/aa-ver0.2_beta
...
- old versions (2.2 - 4.beta)
/imaging/local/spm/aa_svn_new/versions/release-2.2
/imaging/local/spm/aa_svn_new/versions/release-3.0
...
new version (4.0 - 4.1) (based on the latest devel-share branch from GitHub)
/imaging/local/software/AA/release-4.0
/imaging/local/software/AA/release-4.2 - stable
/imaging/local/software/AA/release-4.2_[YYYYMM] - backups on [YYYYMM]
/imaging/local/software/AA/release-4.3 -
recommended
Installing aa
Requirements
- Operating system: Linux only!
- Must-have:
MATLAB: tested on versions >=r2009a.
In CBSU, you need 'r2012b' for parallel computing
Using aa
For a comprehensive summary, please read the latest manuscript. Please, do not forget to cite it when using!
Configuration
Before running aa you need to add the corresponding path to MATLAB Path:
- File/Set Path...
addpath('path_to_aa'); aa_ver4; % adds aa subfolders to the path savepath;
To avoid version conflicts make sure that it is added to the top of the Path. It is even more important that path_to_aa/extrafunctions/spm_mods is on the top (of SPM's paths), so modification to the spm may have effect.
Running
You need a
Optionally, you can also specify your own parameter settings loaded by aarecipe. E.g.: User-specific parameter settings (you may need to change the path pointing to the "parent" xml)
aap = aarecipe('aap_user_parameters.xml');
Features
Parallel computing
- Usage
To enable parallel computing you have to be logged in an appropriate computing machine (login11-14), make sure that our local MATLAB scripts for qsub (/hpc-software/matlab/cbu) is in your MATLAB path, which is there usually, but it is worth checking :), and add the following line to your UMS:
aap.options.wheretoprocess = 'qsub'; % parallel; typical localsingle
Because each thread will load the saved MATLAB path, it is very important that it is set and saved correctly before initiating parallel jobs. See Configuration for more information!
- Monitoring
Handling multiple DICOM sources
- Colon-separated-list (CSL) for rawdatadir:
- The “old” script should work without any changes but the newer ones (based on Recent Update on 2013/09/06) should revert. When multiple sources are defined, use CSL. E.g.:
aap.directory_conventions.rawdatadir = ‘/mridata/cbu:/mridata/csl:/mridata/camcan’; % default at the moment (see aap_parameters_defaults_CBSU.xml)
Support for NIFTI-4D images
- As a default, NIFTI-3D is set. To enable NIFTI-4D, you have to specify it in the UMS:
aap.options.NIFTI4D = 1; % 4D support; typical value 0
Multi-level interlinked reporting
- Levels:
- Study (reporting second-level)
- Subjects (each subjects separately)
- Summaries:
- Subjects
- Registration
- First-level thresholded maps
- Usage
Based on aap structure
aa_report(fullfile(aas_getstudypath(aap),aap.directory_conventions.analysisid));
- If you are in the analysis folder
aa_report;
Garbage collection
- Tidy up unnecessary files - delete any inputs to a module that are not also specified as an output.
- Usage:
Based on aap structure
aas_garbagecollection(aap,true);
- If you are in the analysis folder
aas_garbagecollection(pwd,true);
"Inheritable" parameter settings
There is an option to have site-, user-, and/or study-specific parameter settings containing only the parameters to be overridden (see example).
Non-Standard Modules
- First-level thresholding
- List of significant activation maps
- Overlays on axial slices (T1 MNI)
- Rendering results in 3D
Advanced Features
Input file
It is possible to specify Subjects, Sessions and Events based on a text file. Required options in aap.acq_details.input (see also aap_parameters_defaults_CBSU.xml):
- list: text file:
- Required format:
a CSV file: cells are separated with semicolon
- subcells are separated with "_"
- a header in the first line
- Required columns (may contain more):
- "ID": numbers for identifying subjects
- "FMRI1": cells for the fMRI measurement; it must contain the same number of subcells as the coresponding column header
- first subcell is the CBU volunteer number (without "CBU")
- series numbers are defined in the consecutive subcells with the same order as their names in the coresponding column header
- E.g.:
- As "pure" text:
- ID;Age;Sex;FMRI1_Loc_Con_LDeasy_LDhard
- 02;29;f;90952_7_6_4_5
- Required format:
- selected_sessions: subselection of a subset of series:
E.g. (based on the example above): [3 4] --> LDeasy and LDhard
- referencedirectory_tmpl: a template for path to the folder containing Event files (.mat files in SPM-format)
- Required format:
must contain "*" which will be substituted withID (see above)
- E.g.: '/imaging/ta02/ActionWords/Analysis/E-Prime/DAW*/ref'
The folder should contain the regressor(s) in SPM format (.mat file) with filename format: condition_vol_ID-Session Name.mat.
- E.g. (based on the example above): condition_vol_2-LDeasy.mat
- Required format:
- Then you add these lines to your UMS (based on the example above):
aap.acq_details.input.list='/imaging/ta02/ActionWords/Analysis/Subject_List_Test.csv'; aap.acq_details.input.selected_sessions = [3 4]; aap.acq_details.input.referencedirectory_tmpl = '/imaging/ta02/ActionWords/Analysis/E-Prime/DAW*/ref'; aap = aas_processinput(aap);
Citing aa
Recent Updates
2013/11/25
New feature - first-level model
- Previous implementation allowed for changing only the UNITS. Now, it is possible to fully customize the basis function. The defaults are:
aap.tasksettings.aamod_firstlevel_model.xBF.UNITS = 'secs'; aap.tasksettings.aamod_firstlevel_model.xBF.Volterra = 1; aap.tasksettings.aamod_firstlevel_model.xBF.name = 'hrf'; aap.tasksettings.aamod_firstlevel_model.xBF.length = 32; aap.tasksettings.aamod_firstlevel_model.xBF.order = 1; aap.tasksettings.aamod_firstlevel_model.xBF.T = 17; % number of time bins per TR aap.tasksettings.aamod_firstlevel_model.xBF.T0 = []; % slice at first time bin (empty allows for automatic setting - for both T and T0 - based on slice-time correction)
It means, however, that the old reference aap.tasksettings.aamod_firstlevel_model.UNITS has been deprecated, and aap.tasksettings.aamod_firstlevel_model.xBF.UNITS must be used instead (see UMS).
Fix - subjectname
The most recent update fixes the correspondence of the subjectname, the name you use when adding subject in the User Master Script (USM), and the referring aap setting aap.directory_conventions.subjectoutputformat. It means that subjectname has to be provided according to the setting. The default is 'CBU%06d*', which means that you need to provide the number (as an integer, without CBU), e.g. 90952. Many of you may use part or full name of the directory, which will result an error message most likely upon aamod_evaulatesubjectnames. You have two options:
Either, specify subjectname in UMS according to the setting (see above).
- Or, change the setting:
If you use partial name (e.g. 'CBU090952'), then add this line to the UMS:
aap.directory_conventions.subjectoutputformat = '%s*';
If you use full name (e.g. 'CBU090952_MR09032/20090828_131456'), then add this line to the UMS:
aap.directory_conventions.subjectoutputformat = '%s';
2013/11/21 New features!
aap.directory_conventions.spmtoolsdir: option to specify path for SPM tools which will be taken over when running analysis on the computing cluster. More paths can be specified using the CSL concept similarly to multiple DICOM sources
2013/10/29 New features!
FreeSurfer has been tested
Solving backward-compatibility for handling multiple DICOM sources (see Recent Update on 2013/09/06)
2013/09/06 New features!
DATREL has been tested
Improved monitoring of the parallel computing
Handling multiple DICOM sources (e.g. /mridata/cbu and /mridata/camcan)
Known issue (backward compatibility) SOLVED (see Recent Update on 2013/10/29):
If you want to customize DICOM sources, you need to put your own path to source not into rawdatadir directly (as used to), but into rawdatadir.paths (cell array) or rawdatadir.paths{1} (cellstring).
2013/08/08 Parallel computing is working
See Parallel computing for more information!
Known bugs:
Conventional SPM diagnostic will be missing for:
- aamod_realignunwarp (no report of fieldmaps)
- aamod_coreg_noss
However, FSL diagnostics are unaffected.
2013/08/02 new version v4.1 (latest devel-share branch from GitHub)
merge with GitHub completed
- release-4.1 is now the latest stable
NIFTI-4D support is independent from SPM version. N.B.: Configuration!
2013/06/26 merge with the latest devel-rhodri branch from GitHub
- release-4.0 is now the latest stable
2013/06/06 new feature
NIFTI-4D support added.
2013/05/13 new feature
- Garbage collection tested
- New report summary:
- Head movement: outlier-detection with boxplot
2013/04/24 CBSU Patch/Extension for Version 4 is available
New features:
Handling input file as a list of subjects and sessions
Module for first-level thresholding
Multi-level interlinked reporting:
Known bugs:
Parallel computing is still not working!